{"id":836,"date":"2023-12-12T12:39:00","date_gmt":"2023-12-12T12:39:00","guid":{"rendered":"https:\/\/dev.minervainstitute.fi\/cellular-neurovetenskap\/"},"modified":"2025-01-16T10:28:51","modified_gmt":"2025-01-16T10:28:51","slug":"cellular-neurovetenskap","status":"publish","type":"page","link":"https:\/\/minervainstitute.fi\/sv\/forskningsgrupper\/cellular-neurovetenskap\/","title":{"rendered":"Cellul\u00e4r neurovetenskap"},"content":{"rendered":"

Cellul\u00e4r neurovetenskap<\/h1>\n<\/div>
<\/div><\/div>

Gruppen f\u00f6r cellular neurovetenskap \u00e4r intresserad av de cellul\u00e4ra och molekyl\u00e4ra mekanismer som ligger till grund f\u00f6r inl\u00e4rningen. S\u00e4rskild uppm\u00e4rksamhet har \u00e4gnats \u00e5t dendrittaggar (eng. spines). Dendrittaggar \u00e4r sm\u00e5 utspr\u00e5ng i de neuronala dendriterna d\u00e4r de postsynaptiska komponenterna i excitatoriska synapserna finns i hj\u00e4rnan. Specifika upps\u00e4ttningar av nya taggar uppst\u00e5r n\u00e4r man f\u00e5r nya erfarenheter eller f\u00f6rv\u00e4rvar nya f\u00e4rdigheter, och utg\u00f6r grunden f\u00f6r att beh\u00e5lla dem. D\u00e4rf\u00f6r \u00e4r st\u00f6rningar i regleringen av dendrittaggar involverade i m\u00e5nga neurologiska sjukdomar, fr\u00e5n minnesst\u00f6rningar till psykiatriska sjukdomar. V\u00e5rt m\u00e5l \u00e4r att p\u00e5 ett \u00f6vergripande s\u00e4tt f\u00f6rst\u00e5 hur uppkomsten av dendrittaggar regleras i nervcellerna.<\/p>\n

Ett annat viktigt forskningsobjekt \u00e4r effekterna av perifer inflammation p\u00e5 inl\u00e4rning och minne. \u00c4ven om de direkta effekterna av akut eller kronisk inflammation p\u00e5 hj\u00e4rnan \u00e4r relativt v\u00e4lk\u00e4nda, \u00e4r det oklart om inflammationen orsakar l\u00e5ngvariga f\u00f6r\u00e4ndringar i hj\u00e4rnan. Gruppen unders\u00f6ker de l\u00e5ngsiktiga effekterna av perifer inflammation p\u00e5 kognitiva processer och identifierar de celler som deltar i dessa f\u00f6r\u00e4ndringar.<\/p>\n

I studien anv\u00e4nder gruppen en musmodell (CAIA) f\u00f6r reumatoid artrit. Genom att kombinera beteendetester, bilddiagnostik, cytokinm\u00e4tning och elektrofysiologi analyserar gruppen hur inflammation p\u00e5verkar hj\u00e4rnans funktion p\u00e5 l\u00e5ng sikt.<\/p>\n<\/div><\/div><\/div>

<\/span><\/div>

Gruppledare<\/h3>\n

Pirta Hotulainen, FD, docent
\npirta.hotulainen@helsinki.fi<\/p>\n<\/div>

Gruppmedlemmar<\/h3>\n

Aqsa Jabeen, MSc
\nJaan Korpikoski, MSc
\nMatilda Kuusi, MSc
\nDavid Micinski, MSc
\nVeera Syrj\u00e4niemi, BSc
\nEmilia Toissalo, FM<\/p>\n<\/div><\/div><\/div>

Studien unders\u00f6ker de l\u00e5ngsiktiga effekterna av perifer inflammation p\u00e5 kognitiva processer och identifierar de celler som deltar i dessa f\u00f6r\u00e4ndringar. Resultaten visar ocks\u00e5 hur viktiga de antiinflammatoriska levnadss\u00e4tten \u00e4r f\u00f6r hj\u00e4rnans h\u00e4lsa. Resultaten av studien kommer att bidra till att utveckla b\u00e4ttre behandlingsstrategier f\u00f6r inflammatoriska neurologiska sjukdomar som Alzheimers sjukdom eller long covid<\/p>\n

Metoder<\/u>: Vi anv\u00e4nder en strategi nerifr\u00e5n och upp, d\u00e4r molekyl\u00e4ra mekanismer som l\u00e4rs in i enklare in vitro-system, s\u00e5som provr\u00f6r, fibroblaster eller dissocierade neuroner i hippocampus, som f\u00f6rs upp till mer komplexa system, s\u00e5som organotypiska hj\u00e4rnskivor och in vivo-mushj\u00e4rnan. Avancerade mikroskopitekniker spelar en stor roll p\u00e5 alla niv\u00e5er.<\/p>\n<\/div><\/div><\/div>

<\/span><\/div>

Gruppledare<\/h3>\n

Pirta Hotulainen, FD, docent
\npirta.hotulainen@helsinki.fi<\/p>\n<\/div>

Gruppmedlemmar<\/h3>\n

Aqsa Jabeen, MSc
\nDavid Micinski, MSc
\nEmilia Toissalo, FM<\/p>\n<\/div><\/div><\/div><\/div><\/div>

Utvalda publikationer<\/h3>\n<\/div>
<\/div><\/div>

Micinski D, Hotulainen P. Actin polymerization and longitudinal actin fibers in axon initial segment plasticity. Front Mol Neurosci<\/em>. 2024; 17:1376997.<\/p>\n

Khanal P, Boskovic Z, Lahti L, Ghimire A, Minkeviciene R, Opazo P, Hotulainen P. Gas7 Is a Novel Dendritic Spine Initiation Factor. eNeuro<\/em>. 2023; 10:ENEURO.0344-22.2023.<\/p>\n

Bertling E, Blaesse P, Seja P, Kremneva E, Gateva G, Virtanen MA, Summanen M, Spoljaric I, Uvarov P, Blaesse M, Paavilainen VO, Vutskits L, Kaila K, Hotulainen P*, Ruusuvuori E*. Carbonic anhydrase seven bundles filamentous actin and regulates dendritic spine morphology and density. EMBO Rep<\/em>. 2021; 22:e50145. *PH and ER contributed equally to this work as senior authors<\/p>\n

Abouelezz A, Stefen H, Segerstr\u00e5le M, Micinski D, Minkeviciene R, Lahti L, Hardeman EC, Gunning PW, Hoogenraad CC, Taira T, Fath T, Hotulainen P. Tropomyosin Tpm3.1 Is Required to Maintain the Structure and Function of the Axon Initial Segment. iScience<\/em>. 2020; 23:101053.<\/p>\n

Minkeviciene R, Hlushchenko I, Virenque A, Lahti L, Khanal P, Rauramaa T, Koistinen A, Leinonen V, No\u00e9 FM, Hotulainen, P. MIM-Deficient Mice Exhibit Anatomical Changes in Dendritic Spines, Cortex Volume and Brain Ventricles, and Functional Changes in Motor Coordination and Learning. Frontiers in Mol. Neurosci<\/em>. 2019; 12:276.<\/p>\n

Hlushchenko I, Khanal P, Abouelezz A, Paavilainen VO, Hotulainen P. ASD-Associated De Novo Mutations in Five Actin Regulators Show Both Shared and Distinct Defects in Dendritic Spines and Inhibitory Synapses in Cultured Hippocampal Neurons. Front Cell Neurosci<\/em>. 2018; 12:217.<\/p>\n

Bertling E, Englund J, Minkeviciene R, Koskinen M, Segerstr\u00e5le M, Castren E, Taira T and Hotulainen P. Actin Tyrosine-53-Phosphorylation in Neuronal Maturation and Synaptic Plasticity. J Neuroscience<\/em>. 2016; 36:5299-5313.<\/p>\n

Saarikangas J, Kourdougli N, Senju Y, Chazal G, Segerstr\u00e5le M, Kuurne J, Minkeviciene R, Mattila PK, Garrett L, H\u00f6lter SM, Becker L, Racz I, Hans W, Klopstock T, Wurst W, Zimmer A, Fuchs H, Gailus-Durner V, Hrab\u011b de Angelis M, von Ossowski L, Taira T, Lappalainen P, Rivera O, Hotulainen P. MIM-Induced Membrane Bending Promotes Dendritic Spine Initiation. Dev Cell<\/em>. 2015; 33:644-659.<\/p>\n

Hotulainen P, Hoogenraad CC. Actin in dendritic spines: connecting dynamics to function. J Cell Biol<\/em>. 2010; 189:619-629.<\/p>\n

Hotulainen P, Llano O, Smirnov S, Tanhuanp\u00e4\u00e4 K, Faix J, Rivera C, Lappalainen P. Defining mechanisms of actin polymerization and depolymerization during dendritic spine morphogenesis. J Cell Biol<\/em>. 2009; 185:323-339.<\/p>\n<\/div><\/div><\/div><\/div><\/div>

Finansi\u00e4rer av forskningen<\/h3>\n<\/div>
<\/div><\/div>

Medicinska Underst\u00f6dsf\u00f6reningen Liv och H\u00e4lsa r.f.
\nSigrid Jus\u00e9lius Stiftelse<\/p>\n<\/div><\/div><\/div><\/div><\/div>

F\u00f6lja<\/h3>\n<\/div>
<\/div><\/div>

ResearchGate: Pirta Hotulainen
\nReseacher-ID: B-8874-2015
\nORCID: 0000-0003-0764-8582
\nX: @PirtaHotulainen
\nBluesky: @pirtahotulainen.bsky.social<\/span><\/p>\n<\/div><\/div><\/div><\/div><\/div><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":878,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"100-width.php","meta":{"footnotes":""},"class_list":["post-836","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/minervainstitute.fi\/sv\/wp-json\/wp\/v2\/pages\/836","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/minervainstitute.fi\/sv\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/minervainstitute.fi\/sv\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/minervainstitute.fi\/sv\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/minervainstitute.fi\/sv\/wp-json\/wp\/v2\/comments?post=836"}],"version-history":[{"count":11,"href":"https:\/\/minervainstitute.fi\/sv\/wp-json\/wp\/v2\/pages\/836\/revisions"}],"predecessor-version":[{"id":1608,"href":"https:\/\/minervainstitute.fi\/sv\/wp-json\/wp\/v2\/pages\/836\/revisions\/1608"}],"up":[{"embeddable":true,"href":"https:\/\/minervainstitute.fi\/sv\/wp-json\/wp\/v2\/pages\/878"}],"wp:attachment":[{"href":"https:\/\/minervainstitute.fi\/sv\/wp-json\/wp\/v2\/media?parent=836"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}